Description
Description: This assay employs a two-site sandwich ELISA to quantitate FOLR2 in samples. An antibody specific for FOLR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FOLR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FOLR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FOLR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Folate receptor beta is a member of the folate receptor (FOLR) family, and these genes exist in a cluster on chromosome 11. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and they mediate delivery of 5-methyltetrahydrofolate to the interior of cells.
This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. Multiple transcript variants that encode the same protein have been found for this gene